DNA cloning

DNA cloning technology is the basic terrace for molecular biology .

DNA克隆技术是分子生物学研究中一项重要的技术手段。

DNA Cloning and Sequence Analysis of A TTV Isolated in Wuhan

TTV武汉分离株DNA克隆和序列分析

Human G-CSF Genomic DNA Cloning and its Sequence Analysis

人G-CSF基因组基因的克隆和序列测定

A study of complementary DNA cloning and sequence determination of rabbit type PLA2 with potent bactericidal activity

具强杀菌活性的兔Ⅱ型磷脂酶A2互补DNA克隆及其顺序确定的研究

How to apply it to DNA cloning has become a hotter issue for molecular biologists in the recent years .

如何把同源重组应用到基因克隆中成为近年来生物学家研究的热点。

The a - complementation reaction of & - galactosidase EA and ED was also used in DNA cloning , protein protein interactions monitoring and expressing immunoassays studying .

β-半乳糖苷酶的EA、ED这种α-互补性还被用于分子生物学、蛋白质相互作用的监控、表达免疫分析等方面的研究。

Progress in Development of Large DNA Fragment Cloning Vectors

大片段克隆载体研究进展

Bacterial artificial chromosome ( BAC ) vectors are the secondary system for large DNA fragment cloning .

细菌人工染色体（bacterialartificialchromosome，BAC）是第二代大片段DNA的克隆载体系统。

Optimization of APCR for Unknown DNA Fragment Cloning

DNA未知片段APCR克隆的优化

Construction of Large DNA Fragment Cloning Vectors and Rice Transformation Based on Transformation-competent Artificial Chromosome ( TAC ) Vector

基于TAC多基因转化载体的构建及其转化水稻的研究

An outline of the procedure for introducing a piece of foreign DNA into a cloning vector is shown in Figure 1.3 .

图1.3列出了引出一段外源DNA进入克隆载体的大概步骤。

The main results of this study are as fellow : 1 . Acquisition of a endosperm-specific promoter The Gtl promoter was isolated from rice genome DNA by homology cloning strategy .

本研究目前已取得如下结果：1.胚乳特异性启动子的获得以水稻基因组DNA为模板，通过同源克隆获得水稻谷蛋白基因Gtl的启动子序列pGtl。

Advances in the techniques for extraction of nuclear DNA and isolation and cloning of target genes in fruit trees

果树核DNA提取、目的基因分离与克隆技术研究进展

Androgen regulation of the secreting protein synthesis in Rat Seminal Vesicle was studied by means of DNA recombinant and Molecular cloning technique .

本文报道了应用DNA重组和分子克隆技术研究雄激素与大鼠储精囊分泌蛋白基因的相互作用。

The molecular clones of the A1 gene of Sorghum were isolated from the genomic DNA library of sorghum by the method of genomic DNA cloning , using the cloned A1 gene of Zea mays as the probe .

实验采用基因组克隆的方法，用已经克隆的玉米A1基因作探针，从高粱基因组DNA文库电分离克隆出高粱的A1基因克隆。

Through researching the pattern of DNA methylation , we found that there were many abnormal DNA methylation patterns in cloning , which might be the primary reasons for inducing early death of cloned embryos and developmental abnormalities in cloning .

通过对DNA甲基化模式的研究发现，克隆动物中存在着异常的DNA甲基化状态，而这些异常的DNA甲基化模式可能就是导致克隆胚早期死亡以及克隆动物发育畸形的主要原因。